By Richard Axel (Eds.)
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E. they continued to produce zygotes formed by mating between siblings derived from the zygote. On the other hand, zygotes formed at the four-cell stage of a. spore clones had the normal a7a phenotype (no zygotes formed between cells derived from the zygotes). In a separate experiment, ja spores were allowed to switch to o^, and zygotes from these cells were analyzed. These zygotes b e haved like the zygotes derived from spores. /a HO /HO HMa/HMa HMa"" /HMa" diploid, whether initially ji or ja, can give rise to cells with a phenotype of an a" mating type locus (Figure 4 ) .
The + transformation of his4-912 to H I S 4 shows that pYeHis4 + contains all of the information encoded at H I S 4 . FIGURE 2 A Β C 912 864 —χ x 1176 χ 34 Step (3) (2) (10) Figure 2. The his4 region of yeast. The sites of mutation and his4-912 ( A " B " C " ) , 864 ( C ~ ) , 1176 ( C ~ ) , and 34 (an A~) deletion. 48 4. A L B E R T H I N N E N et al. Integration of pYeHis4 Occurs on Both Sides of His4. There are two possible orientations of the transforming sequences with respect to the recipient sequences (see F i g .
Genetika 9, 8 2 . K l a r , A . J. , and Fogel, S. (1977). Genetics 8 5 , 407. , and Oshima, Y. (1978). Genetics 8 8 , s37. Haber, J. E . , and Garvik, B. (1977). Genetics 8 7 , 33. Kozhina, T. N. (1977). R. ). 26 24. 25o 26. 27. 28o 2. L I N D L E Y C. , and Manney, T. R. ( 1 9 7 4 ) . Genetics 7 6 , 2 5 5 0 Kassir, Υ . , and Simchen, G, ( 1 9 7 6 ) . Genetics 8 2 , 1 8 7 . Oshima, Υ . , and Takano, I. ( 1 9 7 1 ) . Genetics 6 7 , 3 2 7 . Strathern, J. N . , and Hicks, J. , in preparation. E g e l , R.
Eucaryotic Gene Regulation by Richard Axel (Eds.)