New PDF release: Cytogenetics of Aneuploids

By Gurdev S. Khush

ISBN-10: 0124062504

ISBN-13: 9780124062504

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Additional info for Cytogenetics of Aneuploids

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However, in the diploid species where the telotrisomics are most useful for cytogenetic analysis, monosomics in general are not viable, and telocentrics cannot be obtained as in the polyploid species. Nevertheless, monotelodisomics for at least those arms of the chromosome complement whose deficiency is tolerated at the sporophytic level may be induced by radiation or chemical mutagenesis. Thus whole arm deficiencies for fifteen of the twenty-four arms of the tomato chromosome complement are tolerated at the diploid level (Khush and Rick, 1966b, 1968a), and terminal deficiencies for at least eleven of these arms have been induced by radiation treatment.

1936) by the following procedure. A secondary trisomic 2/1 + 3-3 was rendered homozygous for prime type 4 by continuous backcrossing to the latter as the male parent. Secondaries regularly throw primaries in their progenies (Chapter 4). Therefore, among the offsprings of the secondary 2/2 + 3-3 homozygous for the 3-21 and 4-22 chromosomes, a In + 3-21 appeared through segregation. This was a primary 26 2. SOURCES OF TRISOMICS trisomic of prime type 4. By backcrossing to the standard line 1A, it was made homozygous for 3 · 4 and 21-22 chromosomes and thus became a desired tertiary trisomic of the standard line.

Because of their usefulness as a source of secondary trisomics, monoisodisomics were sought using marker genes whose arm locations were known (Khush and Rick, 1967d). According to this method, stocks homozygous for recessive markers of those arms whose deficiencies are tolerated were pollinated with irradiated pollen carrying normal alleles. The ¥1 progenies were grown, and plants showing the recessive phenotype were examined cytologically. Monoisodisomics were included in these pseudodominant plants.

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Cytogenetics of Aneuploids by Gurdev S. Khush

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