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By Yasuo Mukohata

ISBN-10: 0125110502

ISBN-13: 9780125110501

ISBN-10: 0323149383

ISBN-13: 9780323149389

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1977). In "Biochemistry of Membrane Transport (Semenza, G. and Carafoli, E. ), FEBS-Symposium No. 352. Springer-Verlag, Berlin, Heidelberg, New York. CATION FLUX ACROSS BIOMEMBRANES INTERACTION OF INORGANIC PHOSPHATE WITH Na + , K+-ATPase: EFFECT OF P^ ON THE K + DEPENDENT HYDROLYSIS OF P-NITROPHENYLPHOSPHATE1 Kazuya Taniguchi Hideaki Tazawa Terufusa Tamaki Shoichi Iida Department of Pharmacology School of Dentistry Hokkaido University Sapporo Japan I. ,1975). Treatment of K + sensitive phosphoenzyme formed from Pj_ with high concentration of Na + developed sensitivity to ADP with concomitant loss of sensitivity to K + and synthesis of ATP (Taniguchi and Post 1975).

Saturation curve of ouabain-sensitive 2 2 Na+ binding. 26 mM. 2 nmol-mg"l. e. the ouabain-insensitive 2 2 N a + binding plus unbound 2 2 N a + contained in the water space of the precipitate, (·) was also plotted. Fig. 4. Scatchard type plot of ouabain-sensitive 2 2 Na+ binding. The data of ouabain-sensitive 2 2 N a + binding in Fig. 3 were plotted for Scatchard type analysis (O). 07 mM. 36 Hideo Matsui and Haruo Homareda Fig. 5. Simultaneous measurement of 22Na + binding and 4 2 K binding by titration of K+ against Na + .

Biol. Chem. 251:4794. 18. , Homareda, H. and Kimimura, M. (1977). Biochem. Biophys. Res. Commun. 75:373. 19. F. (1977). Anal. Biochem. 83:416. 20. , Homareda, H. and Matsui, H. (1977). Biochim. Biophys. Acta 482:185. 21. Matsui, H. and Schwartz, A. (1968). Biochim. Biophys. Acta 151:655. 22. Scatchard, G. (1949). Ann. Y. Acad. Sei. 51:660. 23. D. and Eilam, Y. (1973). Proc. Natl. Acad. Sei. A. 70:275. 24. , Streckenbach, B. and Dittrich, F. (1974). Ann. Y. Acad. Sei. 242:203. 25. , Taniguchi, K.

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Cation Flux Across Biomembranes by Yasuo Mukohata


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