Download e-book for kindle: Bioprocess Parameter Control by A. Fiechter (auth.)

By A. Fiechter (auth.)

ISBN-10: 0387135391

ISBN-13: 9780387135397

ISBN-10: 3540135391

ISBN-13: 9783540135395

ISBN-10: 3540390049

ISBN-13: 9783540390046

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5 ml * Solution, mg 1-1 A final e x a m p l e o f t h e i m p o r t a n c e o f c o r r e c t m e d i u m d e s i g n is t h e a p p l i c a t i o n o f d e f i n e d b i o s y s t e m s f o r t e s t i n g a e r a t i o n a n d a g i t a t i o n h a r d w a r e . T h e b a s i c idea in s u c h a n a p p r o a c h is t h e fact t h a t c h e m i c a l l y d e f i n e d m e d i a s u p p o r t biologically defined regulation of growth and product formation. Variation of medium concent r a t i o n s (So) p r o v i d e s t h e p o s s i b i l i t y o f c o r r e c t l y a d j u s t i n g t h e b i o s y s t e m to t h e m a s s t r a n s f e r c a p a c i t y o f t h e b i o r e a c t o r (Fig.

In E. coli interferon production was strongly bound to growth and thus depended primarily on fast balanced growth behaviour 52) Figure 22 shows chemostat data obtained with a wild B-type strain o f E . coli and a K 12-strain genetically engineered for interferon production. It is interesting to note that the acetic acid formation observed in these strains seems to reflect a constitutive situation. Later experiments have shown, however, that as in the case with yeasts, restrictions o f trace element delivery to the cell were responsible for the secretion of C2-compound.

E. coli, strain B/r, complex medium containing casamino acids plus some yeast extract (see Table 12). In the defined medium growth behaviour is similar to the values obtained with the complex medium, b. E. coli, strain KI2, D1; Complex medium with glucose as a C-source (Table 12) 52~ Maximum specific growth rates are similar as with the defined medium of Table 12 54 A. The modified medium (first column) effected regulatory effects which could be poorly interpreted. Extensive study of the nutrient demand yielded in the composition given in the second column Table 13.

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Bioprocess Parameter Control by A. Fiechter (auth.)


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