By Gilson Khang, Moon Suk Kim, Hai Bang Lee
Tissue engineering has been well-known as delivering another strategy to whole-organ and tissue transplantation for diseased, failed, or malfunctioned organs. To reconstruct a brand new tissue through tissue engineering, the next triad elements are wanted: (1) cells that are harvested and dissociated from the donor tissue; (2) biomaterials as scaffold substrates within which cells are connected and cultured, leading to implantation on the wanted web site of the functioning tissue; and (3) development elements which advertise and/or hinder telephone adhesion, proliferation, migration, and differentiation. of those 3 key parts, scaffolds play a serious position in tissue engineering. This well timed e-book specializes in the guidance and characterization of scaffold biomaterials for the appliance of tissue-engineered scaffolds. extra importantly, it serves as an experimental guidebook at the standardization of the fabrication method and characterization of scaffolding know-how.
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Extra info for A Manual for Biomaterials/Scaffold Fabrication Technology
2 Procedure • Ice particulates are prepared by spraying deionised water into liquid nitrogen [Fig. 1(a)]. • The sizes of the ice particulates are controlled by the desireable sieving [Figs. 1(c)]. g. methylene chloride or chloroform) and cooling the solution to −20°C. • Ice particulates are added to the precooled polymer solution. • The dispersion is gently vortexed [Fig. 1(d)]. • It is then poured into a precooled designed mould [Fig. 1(e)]. • Subsequently, the mould with dispersion is frozen by placing in low temperature [Fig.
Qxd 5/12/2007 10:48 AM Page 29 FA Characterisations 29 Fig. 3 Image of porous 3D scaffolds fabricated by the ice particle– leaching method. Fig. 4 Pore sizes of PLGA scaffolds fabricated by the ice particle– leaching method with different ratios of polymer and ice. • Their pore structures are analysed by the mercury intrusion method (Fig. 4). 1. 1 Preparation of PLGA scaffolds with different ratios of polymer and ice. 0 Fig. 5 SEM pictures of PLGA scaffold fabricated by the ice particle– leaching method.
Qxd 5/17/2007 8:18 PM Page 7 FA Fabrication and Characterisation for Scaffolds 7 Table 1. The fabrication methods of scaffolds for tissue engineering. Mechanism Method Remark Leaching method Solvent-casting/salt-leaching method Ice particle–leaching method Gas-foaming/salt-leaching method Gel-pressing method Chapter Chapter Chapter Chapter A B C D Microsphere method Biodegradeable microsphere Macroporous bead Particle-aggregated scaffold Chapter E Chapter F Chapter G Phase separation method Freeze-drying method Thermally induced phase separation Centrifugation method Chapter H Chapter I Chapter J Injectable gel Polyphosphazene gel Chapter K Acellular scaffold Decellularisation process Chapter L Keratin scaffold Self-assembled process Chapter M Fibre-spinning method Nanofibre electrospinning process Microfibre wet spinning process Nonwoven PGA fibre Chapter N Chapter O Chapter P Printing and prototyping method Inkjet printing process Melt-based rapid prototyping Chapter Q Chapter R Functional scaffold Growth factor release process Chapter S Ceramic scaffold Sponge replication method Simple calcium phosphate coating method Chapter T Chapter U This book introduces detailed protocols for 21 different types of manufacturing methods for scaffolds in Chapters A–U.
A Manual for Biomaterials/Scaffold Fabrication Technology by Gilson Khang, Moon Suk Kim, Hai Bang Lee